Excellence, Innovation, and Collaboration: Key Research at the MHRC
This past April, we partnered with InsideScientific and the Muscle Health Research Centre (MHRC) at York University, a one-of-a-kind facility that fosters an interdisciplinary approach to the study ...
Sensory Encoding by Muscle Spindle Afferents
This publication review highlights Aurora Scientific instruments that can facilitate the study of sensory encoding by muscle spindle ...
Excellence, Innovation, and Collaboration: Student Research at the MHRC
This past April, we partnered with InsideScientific and the Muscle Health Research Centre (MHRC) at York University, a one-of-a-kind facility that fosters an interdisciplinary approach to the study ...
Functional Recovery of the Musculoskeletal System Following Injury – Leveraging the Large Animal Model
Watch Dr. Sarah Greising discuss the current pathophysiologic understanding of the skeletal muscle remaining following traumatic musculoskeletal ...
Excellence, Innovation, and Collaboration: A Day at the MHRC with Arthur Cheng
Interviewing Dr. Arthur Cheng at the Muscle Health Research Centre (MHRC), York University, an innovative research centre that facilitates the interdisciplinary study of muscle biology and the ...
Excellence, Innovation, and Collaboration: A Day at the MHRC with Chris Perry
Interviewing Dr. Christopher Perry at the Muscle Health Research Centre (MHRC), York University, an innovative research centre that facilitates the interdisciplinary study of muscle biology and the ...
Talking Real Science with Chris Perry
Christopher Perry, PhD, an Associate Professor at York University with the School of Kinesiology & Health Science joined us to talk in detail about his career path that led him to study regulation of ...
Attenuating Muscle Disease Pathology
This publication review discusses how Aurora Scientific muscle physiology products are used in muscular dystrophy preclinical ...
Mitochondrial Bioenergetics and Muscle Function
Highlighted in this publication review are systems available from Aurora Scientific that have aided studies of mitochondrial bioenergetics and muscle function in various research areas, including ...
Myofilament Mechanics in Cardiac and Skeletal Muscle
This publication review highlights how Aurora Scientific offers technologies and instruments for facilitating mechanistic ...
Modelling Exercise-Induced Muscular Changes Using Dynamic Contractions
This publication review highlights how some of our instruments have been used to study the morphological characteristics and function of muscle, as well as myocellular signaling, in rodent models of ...
Mitigating Neuromuscular Deficits
This publication review highlights how several researchers have recently employed our instruments and technologies to better understand these mechanisms and uncover new therapeutic avenues for a ...
Best of 2021: Muscle Physiology
This publication review summarizes some of the best recent articles that fall under our Muscle Physiology ...
Musculoskeletal Complications of Cancer and its Treatments
In this webinar Dr. Bonetto presents evidence that bone preservation directly impacts muscle size and function in cachexia, thus also contributing to unraveling novel pathogenetic mechanisms and ...
How to calibrate an Aurora Scientific High Speed Length Controller using HVSL
Our 315D & 322D models of High-Speed Length Controllers are routinely used by researchers studying small tissue samples such
Biomechanical Properties of GI Smooth Muscle
Image courtesy of Siri et al. (2019). Disorders like gastroparesis, gastroesophageal reflux disease (GERD), and
Targeted genome editing in vivo corrects a Dmd duplication restoring wild-type dystrophin expression
Many rare inheritable diseases often result from duplication mutations. Unfortunately, there is difficulty finding animal models that accurately represent these
Profiling age-related muscle weakness and wasting: neuromuscular junction transmission as a driver of age-related physical decline
Physiological changes that lead to skeletal muscle dysfunction and atrophy during aging are poorly understood and thus therapeutic interventions which
Efficient precise in vivo base editing in adultdystrophic mice
Duchenne muscular dystrophy (DMD) is a muscular disease caused by alterations in dystrophin expression, which has a functional role in muscle force transmission and sarcolemma stability. As such, a loss of dystrophin can lead to muscle weakening and wasting, cardiomyopathy, and several other health conditions. This study focuses on the efficacy of using NG-targeting base editors to restore dystrophin levels in mdx4cv mice. Five weeks following base editor AAv9-iNG injection, mdx4cv mouse hearts exhibited increased levels of dystrophin. Furthermore, approximately 42% of cardiomyocytes became dystrophin-positive following systemic AAV9-iNG treatment, whereas dystrophin expression remained almost completely absent in the control group. A long-term analysis conducted at the 10-month mark showed that dystrophin was almost completely rescued in mdx4cv mouse hearts. In addition to this, dystrophin rescue was also observed in skeletal muscles, including the gastrocnemius and diaphragm, of mdx4cv mice treated with AAV9-iNG. Long-term analysis following intravenous administration of AAV9-iNG also showed almost complete dystrophin restoration (95% of wild type level) in mdx4cv hearts. Through trichome staining analysis, the increased percentage of fibrotic areas seen in the muscle of mdx4cv mice was significantly reduced in those treated with the base editor. Lastly, to determine if AAV9-iNG could improve muscle function, the authors used Aurora’s 1300A 3-in-1 Whole Animal System to measure the maximum plantarflexion tetanic torque during supramaximal electric stimulation of the tibial nerve. The results showed that mdx4cv mice produced significantly less torque compared to wild type mice. Moreover, a significant increase in tetanic torque was observed in AAV9-iNG treated mdx4cv mice. These findings underscore the importance of optimized base editing techniques and their potential use in treating monogenic diseases such as DMD.