Taking CSA measurements using prisms
Variability in fiber size is to be expected when measuring single fiber mechanics so it is important to accurately measure fiber cross-sectional area in order to calculate specific force. Specific force (sFo) is the amount of force generated (mN) per mm2 of fiber (CSA). By using CSA, you can compare the inherent capacity to generate force of each fiber, regardless of size (hypertrophy or atrophy). The 802D has two built in prisms that allow a side view of the fiber (one for inverted, one for dissecting) to properly measure fiber width in both planes as we know that the cross section of a fiber is more accurately modelled by an ellipse rather than a circle (Figure 10). Neither the prism, nor HVSL can measure the dimension of each axis of the elipse on its own. For more details, check out the JoVE article here.
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