1500A
Isolated Muscle System – Microscope Mountable
The Isolated Muscle Systems (1500A, 1510A and 1530A) are highly integrated, turnkey systems providing physiology researchers a simple way to control and measure intact muscle and physiological small tissue samples.
With numerous configuration options, these temperature-controlled systems have the versatility to precisely measure force and length of an array of small tissue types. From engineered tissue constructs to zebrafish, the 1500A system is the serious choice for functional muscle measurements. The glass bottom bath chamber with integrated stimulation electrodes and perfusion permit imaging of the sample while maintaining tissue viability. The apparatus includes XYZ micrometer stages with built-in mounts for our 400C series of force transducers, high-speed length controllers (315/322D), and our optional Dual-Mode lever systems (300E). The standard system configuration includes everything required to start testing immediately including apparatus, length controller, force transducer, stimulator, data acquisition hardware and our unique real-time Linux control and analysis software.
The force transducer is attached to the muscle through a unique slot in the end of the bath. This provides an ideal configuration for artificial muscle constructs or oxygen consumption studies that require the chamber to be sealed with an available lid. The 1500A system works with most inverted microscope, allowing integration of sarcomere length and biofluorimetry measurements. Pair your 1500A with our FluoroTrack and HVSL/VSL video sarcomere length modules for force, calcium and sarcomere length measurements all synchronized in real time. These advanced features allow researchers to completely characterize small intact muscle tissue performing all of the standard measurements including twitch, tetanus, fatigue, length-tension, force-frequency, force-velocity, stiffness and work loops.
Signal acquisition and data management is handled by our software, including standardized protocols, which transform complex experiments into simple and straightforward measurement. A stand is included with each system for easy storage and adaptation to a standard stereo or inverted microscope.
System Variants
1510A: Isometric Force only variant
1530A: Dual-Mode lever system variant
Stories of Success
801C – Ability to Assess Oxygen Consumption/Customization
CHALLENGE
In 2008 Dr. Carter Ralphe at the University of Wisconsin was attempting to measure force and contractility in his tissue constructs in an oxygen sealed environment. Without the time to develop a custom solution, Dr. Ralphe sought help from equipment suppliers. Unfortunately, he wasted a lot of time and money trying equipment from other manufacturers that didn’t work. Although another manufacturer finally refunded his money it took more time and effort to get them to agree to take back their product.
SOLUTION
Dr. Ralphe consulted with Aurora Scientific for the design of a customized oxygen sealing lid that would work with a modified version of our 801C. The redesign allowed the unit to function in oxygen consumption mode or in a regular bath configuration. The customized chamber also allowed easy integration of Aurora Scientific’s industry leading 400 Series transducers which had the sensitivity required for the small and delicate constructs to be tested.
RESULTS
With this instrument, Dr. Ralphe was able to successfully complete his experiments and go on to publish influential results. He now has two units, expanding his research capabilities. Dr. Ralphe has also recommended the device to other researchers who, themselves, have gone on to publish. The 801C-300 has since become essentially a standard modification and a part of our 1500A series of small intact muscle test systems.
Select References
- Norden, Diana M. et al. “Tumor growth increases neuroinflammation, fatigue and depressive-like behavior prior to alterations in muscle function.” Brain, Behavior, and Immunity (2015) DOI: 10.1016/j.bbi.2014.07.013
- Tangney, Jared R. et al. “Timing and magnitude of systolic stretch affect myofilament activation and mechanical work.”” American Journal of Physiology-Heart and Circulatory Physiology (2014) DOI: 10.1152/ajpheart.00233.2014
- Powers et al. “Subcellular Remodeling in Filamin C Deficient Mouse Hearts Impairs Myocyte Tension Development during Progression of Dilated Cardiomyopathy” International Journal of Molecular Sciences (2022) DOI: 10.3390/ijms23020871
- Schick et al. “Reduced preload increases Mechanical Control (strain-rate dependence) of Relaxation by modifying myosin kinetics” Archives of Biochemistry and Biophysics (2021) DOI: 10.1016/j.abb.2021.108909
- Schick et al. “Reduced preload increases Mechanical Control (strain-rate dependence) of Relaxation by modifying myosin kinetics” Archives of Biochemistry and Biophysics (2021) DOI: 10.1016/j.abb.2021.108909
- Zuo, Li, Leonardo Nogueira, and Michael C. Hogan. “Reactive oxygen species formation during tetanic contractions in single isolated Xenopus myofibers.” Journal of Applied Physiology (2011) DOI: 10.1152/japplphysiol.00398.2011
- Zhang et al. “Maturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation” Acta Biomaterialia (2017) DOI: 10.1016/j.actbio.2016.11.058
- Zuo, Li et al. “Low Po2 conditions induce reactive oxygen species formation during contractions in single skeletal muscle fibers.” American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2013) DOI: 10.1152/ajpregu.00563.2012
- Gharanei, Mayel “Investigation into the cardiotoxic effects of doxorubicin on contractile function and the protection afforded by cyclosporin A using the work-loop assay.” Toxicology in Vitro (2014) DOI: 10.1016/j.tiv.2014.01.011
- Laurila et al. “Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy” Science Advances (2022) DOI: 10.1126/sciadv.abh4423
System Components
315D/322D: High-Speed Length Controllers
The 315D/322D High-Speed Length Controllers give physiologists the ability to control and measure length of single cells, fibers and whole muscle with ease.
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400C: Force Transducers
The 400C series of force transducers is the next generation of our widely used 400A and 400B series. The 400C series enables contractile measurements from a variety of muscle types and sizes and are designed to meet the needs of muscle researchers.
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300E: Dual-Mode Muscle Levers
Seamless, flexible control of force and length, with the unique ability to measure one or both in a single and convenient lever system.
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600A: Real-Time Muscle Data Acquisition and Analysis System
The 600A Digital Controller serves to integrate components and provide the researcher control of system operations, data collection and signal analysis.
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701C: Electrical Stimulator
Our high-powered, bi-phase stimulator designed for muscle researchers conducting the most demanding field stimulation protocols.
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801C: Small Intact Muscle Apparatus – Microscope Mountable
Highly integrated apparatus, optimally designed to test small, intact muscle contractility
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902A: Laser Diode Sarcomere Length
A simple, easy to use laser diode system designed for measuring sarcomere length in thicker muscle fibres
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950A: Calcium Fluorescence System
The 950A calcium fluorescence system enables physiology researchers to easily measure intracellular calcium along with tissue length, force and sarcomere length.
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608C: LCD Monitor
A 22” widescreen LCD monitor providing high quality visualization of data collection
Resources
Parts & Accessories
| Model # | 1500A |
|---|---|
| 900B | Video Sarcomere Length (VSL) measurement system includes camera and VSL software that integrates with the 600A Digital Controller. The included USB camera operates at a frame rate of 80 frames per second (FPS) for full frames and up to a maximum of 1000 FPS for reduced frame sizes. The camera mounts via a C-mount adapter (not provided). |
| 901B | High-Speed Video Sarcomere Length (HVSL) measurement system includes camera and HVSL software that integrates with the 600A Digital Controller. The included Gigabit Ethernet camera operates at a frame rate of 300 frames per second (FPS) for full frames and up to a maximum of 4700 FPS for reduced frame sizes. The camera mounts via a C-mount adapter (not provided). |
| 902A | Simple laser diffraction instrument consisting of a laser diode assembly and a SL target assembly. The laser diode mounts to a microscope port via a C-mount adapter (not provided). The SL target assembly mounts to the apparatus. The laser shines through an empty objective port on the microscope turret and passes through the tissue. The sarcomeres in the tissue diffract the laser and form a diffraction pattern on the SL target thus indicating the Sarcomere Length. The desired spacing is set visually and no recording is made. |
| 955A | Ratiometric calcium software which controls, records and analyses calcium transients. Integrates with 600A and VSL/HVSL to provide a complete software package which provides measurement and control of length, force, sarcomere length and calcium in a single file. |
| 830A | Half-rack adapter that allows half-rack instruments to be mounted in standard 19” racking. Works with models: 200B, 315C/322C, 400A series, 701C and 825A. |
| 831A | Heavy gauge, steel desktop or shelf-top rack, with standard 19” width for any Aurora Scientific Instruments. Available in various heights. (6U high for 1200A and 1300A, 5U high for 1400A, 1500A and 1600A systems). |