801C: Small Intact Muscle Apparatus – Microscope Mountable
The 801C small intact muscle test apparatus provides physiology researchers with a simple method of measuring small tissue muscle mechanics. The novel chamber design features a glass bottom with integrated stimulation electrodes and perfusion which permits imaging of the sample while maintaining tissue viability. The open top, horizontal design also permits a sealing lid to be mounted in place for oxygen consumption or hypoxia studies.
The apparatus includes mounts for an Aurora Scientific high-speed length controller (315/322D) and a precision force transducer (400C series). Various sized baths and bath configurations are available which provides the ideal solution for researchers seeking to study different muscle or tissue units and samples. From tissue constructs to zebra fish the 801C is the serious choice for functional muscle measurements.
The force transducer is attached to the muscle through a unique slot in the end of the bath. This provides an ideal configuration for artificial muscle constructs or oxygen consumption studies that require the chamber to be sealed with an available lid. The bath is optimized for smooth perfusate flow and includes an overflow area on either side of the bath to provide good access to the tissue while minimizing the chance of overflow from the bath.
Temperature of the test plate can be controlled from 4 to 40°C within +/- 0.1°C using the supplied Aurora Scientific 825B thermoelectric controller. The 825B also contains a low-noise thermocouple thermometer with both a digital readout and a voltage output. An Aurora Scientific model 826A Water Circulator is also included.
Components
825B Thermocouple Meter / TEC Controller:
Thermometer and TEC Controller controls bath temperature from 0 to 40°C
826A Water Circulator:
Water circulator that provides cooling for 801C, 802D, 803B apparatus. Includes water reservoir, pump and radiator with fan
Variants
Bath Size Variants:
801C-400 (400µL bath)
801C-1900 (1900µL bath)
Isometric Variants:
801C-400-TP (400µL bath with tissue post)
801C-1900-TP (1900µL bath with tissue post)
300E Dual-Mode Muscle Lever Variant:
801C-300 (1900µL bath with mounts for 300E)
No Peltier Temperature Control Variants:
801N-400 (400µL bath, no 825B or 826A)
801N-1900 (1900µL bath, no 825B or 826A)
Stories of Success
801C – Ability to Assess Oxygen Consumption/Customization
CHALLENGE
In 2008 Dr. Carter Ralphe at the University of Wisconsin was attempting to measure force and contractility in his tissue constructs in an oxygen sealed environment. Without the time to develop a custom solution, Dr. Ralphe sought help from equipment suppliers. Unfortunately he wasted a lot of time and money trying equipment from other manufacturers that didn’t work. Although another manufacturer finally refunded his money it took more time and effort to get them to agree to take back their product.
SOLUTION
Dr. Ralphe consulted with Aurora Scientific for the design of a customized oxygen sealing lid that would work with a modified version of our 801C. The redesign allowed the unit to function in oxygen consumption mode or in a regular bath configuration. The customized chamber also allowed easy integration of Aurora Scientific’s industry leading 400 Series transducers which had the sensitivity required for the small and delicate constructs to be tested.
RESULTS
With this instrument, Dr. Ralphe was able to successfully complete his experiments and go on to publish influential results. He now has two units, expanding his research capabilities. Dr. Ralphe has also recommended the device to other researchers who, themselves, have gone on to publish. The 801C-300 since become essentially a standard modification and a part of our 1500A series of small intact muscle test systems.
Select References
- Norden, Diana M. et al “Tumor growth increases neuroinflammation, fatigue and depressive-like behavior prior to alterations in muscle function.” Brain, Behavior, and Immunity (2015) DOI: 10.1016/j.bbi.2014.07.013
- Tangney, Jared R. et al. “Timing and magnitude of systolic stretch affect myofilament activation and mechanical work.” American Journal of Physiology-Heart and Circulatory Physiology (2014) DOI: 10.1152/ajpheart.00233.2014
- Raval, Kunil K. et al. “Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes.” The Journal of Biological Chemistry (2015) DOI: 10.1074/jbc.M114.628628
- Heden et al. “Mitochondrial PE potentiates respiratory enzymes to amplify skeletal muscle aerobic capacity” Science Advances (2019) DOI: 10.1126/sciadv.aax8352
- Gharanei, Mayel “Investigation into the cardiotoxic effects of doxorubicin on contractile function and the protection afforded by cyclosporin A using the work-loop assay.” Toxicology in Vitro (2014) DOI: 10.1016/j.tiv.2014.01.011
- Munarin et al. “Engineered human myocardium with local release of angiogenic proteins improves vascularization and cardiac function in injured rat hearts” Biomaterials (2020) DOI: 10.1016/j.biomaterials.2020.120033
- de Lange, Willem J. et al. “E258K HCM-causing mutation in cardiac MyBP-C reduces contractile force and accelerates twitch kinetics by disrupting the cMyBP-C and myosin S2 interaction.” The Journal of General Physiology (2013) DOI: 10.1085/jgp.201311018
- Gharanei, Mayel et al. “Doxorubicin induced myocardial injury is exacerbated following ischaemic stress via opening of the mitochondrial permeability transition pore.” Toxicology and Applied Pharmacology (2013) DOI: 10.1016/j.taap.2012.12.003
- Dwenger et al. “Chronic Optogenetic Pacing of Human-Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues” Channelrhodopsin (2020) DOI: 10.1007/978-1-0716-0830-2_10
- de Lange, Willem J. et al “Ablation of cardiac myosin-binding protein-C accelerates contractile kinetics in engineered cardiac tissue.” Journal of General Physiology (2013) DOI: 10.1085/jgp.201210837
Related Products
315D/322D: High-Speed Length Controllers
The 315D/322D High-Speed Length Controllers give physiologists the ability to control and measure length of single cells, fibers and whole muscle with ease.
Learn More
400C: Force Transducers
The 400C series of force transducers is the next generation of our widely used 400A and 400B series. The 400C series enables contractile measurements from a variety of muscle types and sizes and are designed to meet the needs of muscle researchers.
Learn More
300E: Dual-Mode Muscle Levers
Seamless, flexible control of force and length, with the unique ability to measure one or both in a single and convenient lever system.
Learn More
600A: Real-Time Muscle Data Acquisition and Analysis System
The 600A Digital Controller serves to integrate components and provide the researcher control of system operations, data collection and signal analysis.
Learn More
605A: Dynamic Muscle Data Acquisition and Analysis System
Precise, custom software designed for real-time data acquisition, instrument control and data analysis with unique, integrative features. Includes force and SL control capability
Learn More
701C: Electrical Stimulator
Our high-powered, bi-phase stimulator designed for muscle researchers conducting the most demanding field stimulation protocols.
Learn More
825A: Thermocouple Meter / TEC Controller
Thermometer and TEC Controller controls bath temperature from 0 to 40°C
Learn More
901D: High-Speed Video Sarcomere Length
A simple, turn-key system for measuring sarcomere spacing in isolated muscle preparations.
Learn More
902A: Laser Diode Sarcomere Length
A simple, easy to use laser diode system designed for measuring sarcomere length in thicker muscle fibres
Learn More
950A: Calcium Fluorescence System
The 950A calcium fluorescence system enables physiology researchers to easily measure intracellular calcium along with tissue length, force and sarcomere length.
Learn MoreSpecifications
| Bath | 801C-400 | 801C-1900 |
|---|---|---|
| Number | 1 | |
| Volume [µL] | 400 | 1900 |
| Dimensions [mm] | 24 L x 6 W x 3 D | 34 L x 10 W x 6 D |
| Perfusion | 801C-400 | 801C-1900 |
|---|---|---|
| Inlet/Outlet Vinyl Tube Size [IN] | 1/16” ID x 1/8” OD | |
| Inlet/Outlet Stainless Tube Size [IN] | 18AWG | |
| Suction Height Range [mm] | 3.0-6.0 | 6.0-9.0 |
| Thermoelectric Coolers | 801C-400 / 801C-1900 |
|---|---|
| Number | 2 |
| Control Temperature [°C] | 0 – 40 |
| Power [W] | 33 |
| Voltage [V] | 12 |
| Thermocouple Microprobe | 801C-400 / 801C-1900 |
|---|---|
| Model | Physitemp IT-18 |
| Type | T Thermocouple Copper-Constantan |
| Time Constant [s] | 0.1 |
| Construction | 0.064 mm diameter Teflon tube with sealed tip |
| Stimulation Electrodes | 801C-400 / 801C-1900 |
|---|---|
| Number | 2 |
| Type | 90% Platinum, 10% Iridium |
| Dimensions [mm] | 2 W x 0.25 Thick x 47 L |
| General Specifications | 801C-400 / 801C-1900 |
|---|---|
| Weight [kg] | 1.9 (with stand), 1.2 (without stand) |
| Dimensions1 [cm] | 16.5W x 21 L x 6.9 H |
1 = Apparatus without stand