The High-Speed Video Sarcomere Length (HVSL) system provides high-speed, accurate sarcomere length measurement from a live video image. Our 901D HVSL system uses a high-resolution, USB 3.0 camera to provide full-frame images (1280×1024) at up to 300 frames per second. This frame rate increases to 3,000 frames per second when the camera is windowed down to 16 pixel lines.
Our software allows dynamic tracking of sarcomere length within the image which ensures that, as the tissue moves and contracts, the same sarcomeres will always be used for the calculation. Image capture and sarcomere length data can be controlled by our 600A Digital Controller software which runs on a real-time Linux operating system.
Unique Record and Playback functions allow high-speed streaming to disk and permit calculation of sarcomere length from image files. The Analysis function graphically displays sarcomere length and velocity over time.
The software includes a Scope function that provides a scrolling data display of sarcomere length with time. Researchers can accurately and easily record up to 2 hours of sarcomere length data. The Scope display allows freeze-framing at any time. In addition to scrolling through the sarcomere history, Scope also provides SL data logging to disk at rates of up to 1,000 samples per second.
The program includes two computationally accurate and fast length calculation methods, Autocorrelation Function (ACF)/Sine-fit and Fast Fourier Transform (FFT)/curve fit.