602A: CardioLoop Data Acquisition and Analysis System
The 602A CardioLoop System serves to integrate components and provide the researcher control of system operations, data collection and signal analysis. Most importantly, commands and measurements are executed in real-time using a Linux-RTAI operating system. This ensures precise synchronization of equipment and real-time recording. CardioLoop is a powerful new software system specifically designed to perform detailed mechanics experiments on isolated cardiac muscle, including myocardial slices and strips. These experimental protocols are all structured on the basis of continuous pacing, rather than being temporally based, with a fixed duration of stimulation and length changes occurring at a predetermined time. This experimental structure naturally lends itself to twitch based contractions performed to assess basic contractility, however, it also provides a deeper level of functionality and permits a more physiological modelling of the cardiac cycle.
The system includes our 655A real-time CardioLoop software, a custom PC with Linux OS (601B), an on-board 16-bit DAQ card and a 604A signal interface with all required cables.
The unit is capable of controlling and receiving input from all 300 series muscle levers and length controllers, 400C series force transducers, 700 series stimulators and seamlessly connects to all Aurora Scientific Linux-based software products such as 901D HVSL and 955A FluoroTrack. This permits everything from a simple twitch to length-tension, isotonic, cardiac cycle, stiffness, force-velocity and more.
Also included with the system is an extensive library of standardized experimental protocols, making even the most demanding tests and multi-measurement processes straightforward and simple.
Components
601B: Linux PC
603C: 16-bit Data Acquisition Card
604A: Signal Interface
655A: CardioLoop Data Acquisition and Analysis Software
Parts & Accessories
655B: 2nd Copy CardioLoop Software
655C: Upgrade CardioLoop Software
608C: Monitor, 22″ Widescreen LCD
606A: BNC-BNC Cable
607A: BNC Terminator
607B: BNC T
Select References
- Bagni, M. Angela et al “The effects of fatigue and oxidation on contractile function of intact muscle fibers and myofibrils isolated from the mouse diaphragm.” Scientific Reports (2019) DOI: 10.1038/s41598-019-39353-5
- Kazmierczak K. et al. “Slow-twitch skeletal muscle defects accompany cardiac dysfunction in transgenic mice with a mutation in the myosin regulatory light chain.” The FASEB Journal (2019) DOI: 10.1096/fj.201801402R
- Robbins N. et al. “Probenecid Improves Cardiac Function in Patients With Heart Failure With Reduced Ejection Fraction In Vivo and Cardiomyocyte Calcium Sensitivity In Vitro.” Journal of the American Heart Association (2018) DOI: 10.1161/JAHA.117.007148
- Weidner at al. “Impact of lengthening velocity on the generation of eccentric force by slow-twitch muscle fibers in long stretches” Pflügers Archiv - European Journal of Physiology (2024) DOI: 10.1007/s00424-024-02991-4
- Ijpma, Gijs et al. “Human trachealis and main bronchi smooth muscle are normoresponsive in asthma.” American Journal of Respiratory and Critical Care Medicine (2015) DOI: 10.1164/rccm.201407-1296OC
- Hessel et al. “Fast myosin binding protein C knockout in skeletal muscle alters length-dependent activation and myofilament structure” Communications biology (2024) DOI: 10.1038/s42003-024-06265-8
- Siebenaller et al. “Comparing contractile properties within an integrated group of muscles: The abdominal wall” Journal of Biomechanics (2022) DOI: 10.1016/j.jbiomech.2022.111355
- McNamara, James W. et al. “Cardiac myosin binding protein-C phosphorylation regulates the super-relaxed state of myosin.” Proceedings of the National Academy of Sciences (2019) DOI: 10.1073/pnas.1821660116
- Bagni, M. Angela et al “The effects of fatigue and oxidation on contractile function of intact muscle fibers and myofibrils isolated from the mouse diaphragm.” Scientific Reports (2019) DOI: 10.1038/s41598-019-39353-5
- Kazmierczak K. et al. “Slow-twitch skeletal muscle defects accompany cardiac dysfunction in transgenic mice with a mutation in the myosin regulatory light chain.” The FASEB Journal (2019) DOI: 10.1096/fj.201801402R
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Specifications
| System Specifications | 602A |
|---|---|
| Instruments Included in System | 601B – PC with Real-Time Linux 603C – 16-bit Data Acquisition Card 604A – Signal Interface 655A – CardioLoop Data Acquisition and Analysis Software |
| Aurora Scientific Instruments Controlled | 300 series length controllers, 400 series force transducers, 700 series stimulators, 800 series apparatus, 901D HVSL sarcomere length, 950A Fluorescence |
| 601B PC Specifications | 602A |
|---|---|
| Processor [GHz] | Intel i5-10400 CPU 2.9GHz, 6 cores |
| RAM [GB] | 16 |
| Hard Disk [GB] | 500 |
| 603C Data Acquisition Specifications | 602A |
|---|---|
| Analog Input | Number of Channels: 8 Resolution: 16 bits Sampling Rate: 20,000 samples per second |
| Analog Output | Number of Channels: 2 Resolution: 16 bits Sampling Rate: 20,000 updates per second |
| Digital I/O | Number of Ports: 61 Type: TTL |
1 = 6 available on 604A Signal Interface, 24 available on Data Acquisition card
| 604A Signal Interface Specifications |
|
|
|---|---|---|
| Connector | BNC – isolated, male | |
| A/D Inputs | 8: Length In, Force In, Aux 1-6 In | |
| A/D Outputs | 2: Length Out, Force Out | |
| Digital I/O | 6 total: 2 Inputs: Trg In 1, Trg In 2, 4 Outputs: Trg Out 1, Trg Out 2, Stimulator, Inhibit |
| 655A Software Specifications | 602A |
|---|---|
| Operating System | Linux – Debian 11 – PREEMPT RT, real-time, 64-bit |
| Companion Software | 470A, 650A, 655A, 802D, 820A, 901D, 950A |
| Data Channels Recorded | Length, Force, Sarcomere Length, Stimulation Timing, Auxiliary 1, Auxiliary 22 |
| Main Window | Elapsed Time, Time since Last Stimulus, Length and Force Readouts. Display: graph of test results as they are collected. Buttons for: Start Sequence, Start Test, Next Test, Stop Test and Zero Lout. Dropdown menus: File, Edit, Calibrate, Setup, Protocol, Analysis, Scope, Burst and Help. |
| Protocol Functions | Length: Step, Ramp, Square, Sine, Sweep, Sample, Hold Force: Step, Ramp, Square, Sine, Sweep, Sample, Hold, Clamp Sarcomere Length: Step, Ramp, Sample, Hold, Trigger, Track Stimulation: Stimulus, Trigger 1, Trigger 2 Control: Sync Stimulus, Data Enable, Data Disable, Data Burst, Trigger FLA, Bath, Repeat, Stop |
| Sequencer | Allows protocols to be run in a pre-determined manner with control of time between each protocol (skip beats) |
| Scope Data Display | Real-time data output of chosen channels. Displays up to 1 hour of data – zoom function, time base control, pause function |
| Analysis | Per-twitch analysis: force, length, velocity, dF/dt, SL, AUX1, AUX2; work-loop and pressure-volume loop |
| Export Analysis | Creates ASCII data files of the data presented on the Analysis window |
2 = Auxiliary Channels: can be configured to suit, common usages are: Experimental monitors: Temperature, pH, Fluorescence: Numerator, Denominator