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Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

By In JoVE Videos On June 25, 2015


Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. In this article we outline a valid and reliable technique to prepare and test permeabilized skeletal muscle fibers in vitro.

 

Date Published: 6/16/2015, Issue 100; doi: 10.3791/52695

Roche, S. M., Gumucio, J. P., Brooks, S. V., Mendias, C. L., Claflin, D. R. Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers. J. Vis. Exp. (100), e52695, doi:10.3791/52695 (2015).

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