The 1600A Permeabilized Myocyte Test System is designed to simplify the complex testing of contractile properties of skinned myocytes. A large cell mounting area provides ample room for cell attachment. Push button controlled motorized stages simplifies cell attachment to the high-speed servo motor (315C, 322C) and precision force transducer (400 series).
A temperature controlled 8-well bath plate allows for seamless transition of the myocyte between varying calcium concentrations. Direct mounting on an inverted microscope stage allows for observation and sarcomere spacing detection. An optional prism reticule can be included to provide accurate measurement of cell cross section.
With the 1600A, force, length and sarcomere spacing (with optional HVSL/VSL) can all be controlled and measured easily and repeatedly. This permits measurement of simple force-pCa to more complex mechanical characterization of the cell including kTr, length-tension, force-velocity and stiffness.
The system is truly turnkey, including components for data acquisition and analysis as well. Our Digital controller and Software Analysis package (600A) comes complete with a large library of standardized protocols to make the above characterizations straightforward. Choose the 1600A system for performance, precision and progress.
Related Products
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- complete test system for permeabilized myocytes
- temperature controlled 8-well bath plate (sizes from 400µL to 1900µL)
- large cell mounting area and motorized XYZ micro-positioning stages with computerized control
- control and measure force, length and sarcomere spacing (with optional 900B/901C)
- can be integrated with Aurora Scientific 900B/901C Sarcomere Length Software (VSL/HVSL)
- simple mounting to an inverted microscope
- measure force-pCA, kTR, stiffness, length-tension and force-velocity relationships
- optional optical prism included for measurement of cell depth
1600A – Turnkey Solution Aids Characterization of Cardiac Cell Mechanics in Heart Failure
CHALLENGE
Dr. Jonathan Kirk was working as a postdoctoral fellow in Dr. David Kass’ lab at Johns Hopkins University who had acquired his own custom built equipment for testing mechanics of heart failure in skinned cardiomyocytes. When Dr. Kirk secured a faculty position at Loyola University in Chicago, he did not have the means to fabricate his own custom apparatus and stages to characterize cardiomyocyte function. He also needed to find a way to integrate his own stepper motor for small-scale length changes in single cell preparations while measuring and monitoring sarcomere length.
SOLUTION
Aurora Scientific had worked closely with Dr. Kirk and Dr. Kass previously so we knew we would be able to provide him a complete solution for his new lab. The 1600A Permeabilized Myocyte System was the answer as it included our 803B 8-well skinned myocyte apparatus for various calcium concentrations, 3-axis motorized stages and a complete data acquisition and analysis system. Furthermore, Aurora Scientific provided our 400A series high-resolution force transducer and incorporated our video sarcomere length software (VSL) and camera for measuring and monitoring sarcomere spacing.
RESULTS
The permeabilized myocyte system was provided to Dr. Kirk, which was easily mounted on an inverted microscope and combined with motorized stages to deliver precise control of the transducer and motor for cell attachment and testing. Furthermore, the 8-well bath plate allowed for quick determination of force-pCA in these cells. Additionally, our data acquisition software and VSL enabled full integration of Dr. Kirk’s stepper motor for precise length changes, easily monitored using the VSL camera. The 1600A has provided Dr. Kirk with a system that will allow him to characterize mechanics of heart failure and discover the mechanisms involved, leading the way to the development of potentially ground-breaking treatments for humans with this condition.
Models Included With This System
Uniquely designed for fast and accurate length control during dynamic contractile experiments.
Complete, ready-to-use force transducers designed for fast response, high resolution and low compliance measurement of mechanical properties.
A precision, real-time controller and data acquisition system for Aurora Scientific apparatus and measurement devices,
A 22” widescreen LCD monitor providing high quality visualization of data collection
Innovative 8–well plate designed for quick cell attachment and measurement of myocyte mechanical properties.