1600A
Permeabilized Myocyte System – Microscope Mountable
The 1600A Permeabilized Myocyte Test System is designed to simplify the complex testing of contractile properties of skinned myocytes. A large cell mounting area provides ample room for cell attachment. Push button controlled motorized stages simplifies cell attachment to the high-speed servo motor (315C, 322C) and precision force transducer (400 series).
A temperature controlled 8-well bath plate allows for seamless transition of the myocyte between varying calcium concentrations. Direct mounting on an inverted microscope stage allows for observation and sarcomere spacing detection. An optional prism reticule can be included to provide accurate measurement of cell cross section.
With the 1600A, force, length and sarcomere spacing (with optional HVSL/VSL) can all be controlled and measured easily and repeatedly. This permits measurement of simple force-pCa to more complex mechanical characterization of the cell including kTr, length-tension, force-velocity and stiffness.
The system is truly turnkey, including components for data acquisition and analysis as well. Our Digital controller and Software Analysis package (600A) comes complete with a large library of standardized protocols to make the above characterizations straightforward. Choose the 1600A system for performance, precision and progress.

Inverted microscope with Permeabilized Myocyte Apparatus

Eight temperature controlled bath wells

Direct mounting of apparatus on inverted microscope
Stories of Success
Select References
- Alegre-Cebollada, Jorge et al. “S-glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding.” Cell (2014) DOI: 10.1016/j.cell.2014.01.056
- Krysiak et al. “Protein phosphatase 5 regulates titin phosphorylation and function at a sarcomere-associated mechanosensor complex in cardiomyocytes” Nature communications (2018) DOI: 10.1038/s41467-017-02483-3
- Gonçalves-Rodrigues et al. “In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes” Journal of Visualized Experiments (2020) DOI: 10.3791/60427
- Pappritz et al. “Impact of syndecan-2-selected mesenchymal stromal cells on the early onset of diabetic cardiomyopathy in diabetic db/db mice” Frontiers in Cardiovascular Medicine (2021) DOI: 10.3389/fcvm.2021.632728
- Pappritz et al. “Impact of syndecan-2-selected mesenchymal stromal cells on the early onset of diabetic cardiomyopathy in diabetic db/db mice” Frontiers in Cardiovascular Medicine (2021) DOI: 10.3389/fcvm.2021.632728
- Kötter, Sebastian et al. “Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.” The Journal of Cell Biology (2014) DOI: 10.1083/jcb.201306077
- Horiguchi, Hiroshi et al. “Fabrication and evaluation of reconstructed cardiac tissue and its application to bio-actuated microdevices.” IEEE Transactions on NanoBioscience (2009) DOI: 10.1109/TNB.2009.2035282
- Kolijn et al. “Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator” Frontiers in Physiology (2020) DOI: 10.3389/fphys.2020.00345
- Chung, Eunhee and Gary M. Diffee. “Effect of aging on power output properties in rat skinned cardiac myocytes.” The Journals of Gerontology Series A: Biological Sciences and Medical Sciences (2011) DOI: 10.1093/gerona/glr150
- Waddingham et al. “Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model” Journal of Molecular and Cellular Cardiology (2019) DOI: 10.1016/j.yjmcc.2019.10.005
- Hamdani, Nazha et al. “Deranged myofilament phosphorylation and function in experimental heart failure with preserved ejection fraction.” Cardiovascular Research (2013) DOI: 10.1093/cvr/cvs353
- Rivas-Pardo et al. “A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues” Nature communications (2020) DOI: 10.1038/s41467-020-15465-9
System Components
803B: Permeabilized Myocyte Apparatus – Microscope Mountable
Innovative 8–well plate designed for quick cell attachment and measurement of myocyte mechanical properties
Learn More400C: Force Transducers
The 400C series of force transducers is the next generation of our widely used 400A and 400B series. The 400C series enables contractile measurements from a variety of muscle types and sizes and are designed to meet the needs of muscle researchers.
Learn More315D/322D: High-Speed Length Controllers
The 315D/322D High-Speed Length Controllers give physiologists the ability to control and measure length of single cells, fibers and whole muscle with ease.
Learn More600A: Real-Time Muscle Data Acquisition and Analysis System
The 600A Digital Controller serves to integrate components and provide the researcher control of system operations, data collection and signal analysis.
Learn More
608C: LCD Monitor
A 22” widescreen LCD monitor providing high quality visualization of data collection
Resources
Parts & Accessories
Model # | 1600A |
---|---|
900B | Video Sarcomere Length (VSL) measurement system includes camera and VSL software that integrates with the 600A Digital Controller. The included USB camera operates at a frame rate of 80 frames per second (FPS) for full frames and up to a maximum of 1000 FPS for reduced frame sizes. The camera mounts via a C-mount adapter (not provided). |
901B | High-Speed Video Sarcomere Length (HVSL) measurement system includes camera and HVSL software that integrates with the 600A Digital Controller. The included Gigabit Ethernet camera operates at a frame rate of 300 frames per second (FPS) for full frames and up to a maximum of 4700 FPS for reduced frame sizes. The camera mounts via a C-mount adapter (not provided). |
830A | Half-rack adapter that allows half-rack instruments to be mounted in standard 19” racking. Works with models: 200B, 315C/322C, 400A series, 701C and 825A. |
831A | Heavy gauge, steel desktop or shelf-top rack, with standard 19” width for any Aurora Scientific Instruments. Available in various heights. (6U high for 1200A and 1300A, 5U high for 1400A, 1500A and 1600A systems). |