1415A

1415A – Measuring ATPase activity with High Resolution

CHALLENGE

In 2016 Aurora Scientific was approached by Dr. Pieter de Tombe and Dr. Ramzi Khairallah of Loyola University to partner on the development and commercialization of a new apparatus for making measurements of ATP consumption in permeabilized tissue. This initiative was in response to what was perceived to be a growing demand to make these measurements again after long period of sparse publication on the subject, and the disappearance of any commercial offerings for researchers without the means to construct their own system. Price was also a very important consideration as equipment available in the past did not have motorized movement of the bath plate and was equipped with expensive optics for the ATPase portion of the experiment.

SOLUTION

After many prototypes and iterations a version of the apparatus which could fit onto a typical inverted microscope was developed. Aurora was able to leverage their experience with precise motion control from the 802D fiber apparatus to develop a reliable and relatively inexpensive moving chamber while Dr’s de Tombe and Khairallah’s extensive knowledge of optics helped leverage new optical technologies available to achieve good ATP resolution much more cheaply than possible in the past.

RESULTS

We are very proud to present the 804A Permeabilized Fiber ATPase Apparatus which represents a true collaborative effort between industry and academia and the best of the spirit of scientific discovery. We are grateful for the efforts of all parties involved.

Select References

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• Garibaldi et al. “Novel ACTA1 mutation causes late-presenting nemaline myopathy with unusual dark cores” Neuromuscular Disorders (2021) DOI: 10.1016/j.nmd.2020.11.012
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