800C: in-vitro Muscle Apparatus
The 800C in-vitro muscle apparatus is designed for use with any configuration of our 300E and 305E Dual-Mode muscle levers as well as our models 402C and 407C force transducer for isometric measurements only. The apparatus can accommodate a variety of bath volumes from 10mL to 100mL to adapt to a range of muscle sizes from mice, rats and other small animal models. Flexible in design, this apparatus provides a vertical, in-vitro measurement of intact muscle in a tissue bath. The apparatus can be fixed together to create a multi-channel bath apparatus (2 or 4 baths).
The bath is mounted to a vertical translation stage, allowing the researcher to raise and lower the bath for easy access to the tissue during mounting, removal and measurement. The bath is doubled walled which allows for easy temperature control and includes an oxygenating bubbler. A dual fine/coarse micropositioner permits accurate positioning of the tissue and adjustment of resting tension or muscle length.
For the researcher’s convenience, T-slots on the mounting frame allow all clamps to be rearranged and additional instrumentation can be easily added to the extruded profile. Designed for long life, the apparatus is made of corrosion resistant materials, with attention paid to details such as potted electrodes to prevent corrosion.
Models
800C-1 Single Bath in-vitro Muscle Apparatus For Mouse or Rat:
Test a single muscle from a mouse or rat with either a 300E Dual-Mode muscle lever, 305E Dual-Mode muscle lever or 400C Series Force Transducer (isometric only)
Bath volumes available – 10mL, 50mL and 100mL
800C-2 Dual-Bath in-vitro Muscle Apparatus For Mouse or Rat:
Test two muscles from a mouse or rat with any combination of 300E Dual-Mode muscle levers, 305E Dual-Mode muscle levers or a 400C Series Force Transducer (isometric only)
Bath volumes available – 10mL, 50mL and 100mL
Select References
- Segales et al. “Sestrin prevents atrophy of disused and aging muscles by integrating anabolic and catabolic signals” Nature communications (2020) DOI: 10.1038/s41467-019-13832-9
- Gorini et al. “Reversible temporally-specific inhibition of muscle using a light-activated chloride channel” bioRxiv (2018) DOI: 10.1101/398156
- Cosentino et al. “Remodeled eX vivo muscle engineered tissue improves heart function after chronic myocardial ischemia” Scientific Reports (2023) DOI: 10.1038/s41598-023-37553-8
- Llewellyn, Michael E. et al. “Orderly recruitment of motor units under optical control in vivo.” Nature Medicine (2010) DOI: 10.1038/nm.2228
- Ma et al. “Myosin Head Configurations in Resting and Contracting Murine Skeletal Muscle” Internation Journal of Molecular Sciences (2018) DOI: 10.3390/ijms19092643
- Hamoudi et al. “Muscle weakness and selective muscle atrophy in osteoprotegerin-deficient mice” Human Molecular Genetics (2020) DOI: 10.1093/hmg/ddz312
- Zhao et al. “Meningeal P2X7 Signaling Mediates Migraine-Related Intracranial Mechanical Hypersensitivity” The Journal of Neuroscience (2023) DOI: 10.1523/JNEUROSCI.0368-23.2023
- Ottenheijm, Coen A.C. et al. “ltered contractility of skeletal muscle in mice deficient in titin’s M-band region.” Journal of Molecular Biology (2009) DOI: 10.1016/j.jmb.2009.08.009
- Jackson, Janna R., Michael J. Ryan, and Stephen E. Alway. “Long-term supplementation with resveratrol alleviates oxidative stress but does not attenuate sarcopenia in aged mice.” The Journals of Gerontology Series A: Biological Sciences and Medical Sciences (2011) DOI: 10.1093/gerona/glr047
- Gruebele et al. “Long-Stroke Rolling Diaphragm Actuators For Haptic Display of Forces in Teleoperation” IEEE Robotics and Automation Letters (2019) DOI: 10.1109/LRA.2019.2894867
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Learn MoreSpecifications
| Apparatus | 800C-1 | 800C-2 |
|---|---|---|
| Type | Anodized Extruded Aluminum “T” Profile | |
| Tissue Clamp Movement [mm] | ±10 | |
| Travel per Knob Rotation [mm] | Course: 20 Fine: 2.6 |
|
| Bath Movement [mm] | Nominal: 70 Maximum: 160 |
|
| Bath | 800C-1 | 800C-2 |
|---|---|---|
| Type | Radnoti Tissue-Organ Bath with T.N.V Dispenser Tube | |
| Volume [ml] | Small: 50 Large: 100 |
|
| Maximum Muscle Length [mm] | >55 | |
| Bath Dimensions [mm] | Small: 51 OD x 34 ID x 75 H Large: 75 OD x 51 ID x 90 H |
|
| Number of Baths | 1 | 2 |
| Temperature Control | Fully water-jacketed with quick disconnect fittings | |
| Stimulation | 800C-1 | 800C-2 |
|---|---|---|
| Holder Material | Delrin | |
| Type | Pure Platinum Bar | |
| Dimensions [mm] | 2 W x 30 AWG x 50 L | |
| Number of Electrode Pairs | 1 | 2 |
| General Specifications | 800C-1 | 800C-2 |
|---|---|---|
| Operating Temperature [°C] | 0-40 | |
| Weight [kg] | 2.15 | 3.76 |
| Dimensions [cm] | 28.5W x 23.1D x 43.8H | 43W x 23.1D x 43.8H |