809C: in-situ Mouse Apparatus

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The 809C mouse apparatus performs three different tests for measuring muscle properties using a single, easy-to-use platform. In two of these configurations, the apparatus is mounted with an animal and limb plate for measuring contractions in-situ (using a lever arm) or muscle dynamics in-vivo (using a footplate). The third configuration involves the addition of a 25mL horizontal in-vitro bath, enabling contractile measurements in isolated, intact muscle using a lever arm.

The animal platform is temperature-controlled and adjustable to ensure clear access to the animal while positioning the limb. A variety of limb clamps for specific applications and research subjects are also available, creating greater ease of use during examination.

The 809C is compatible with our 300C series of muscle levers . All models are mounted on slides to position the lever easily with respect to the limb. Motor mounts also provide a fine translation stage to properly set resting tension of the muscle.

  • in-vitro, in-situ and in-vivo muscle experimentation in one platform

  • designed specifically for mouse applications

  • simple to switch between configurations

  • flexible limb clamping

  • adjustable animal platform with option for temperature control using a circulating bath

  • dual coarse/fine translation stage

  • T-slot design for flexibility and expansion

  • corrosion resistant materials

Variant

in-vitro Bath Variant:
809C-IV in-vitro Mouse Apparatus:
Perform in-vitro experiments on intact mouse muscle in the 25 mL horizontal bath

Stories of Success

809C – in-vitro Configuration Sheds Light on the Relationship Between Muscle and Nerve

CHALLENGE

In 2010, Dr. Katherine Wilkinson, then a postdoc at Emory University, approached us about equipment for testing EDL muscle from mice. Specifically, Dr. Wilkinson was interested in sensory neurons innervating the skeletal muscle and how these neurons fire in response to various mechanical, chemical and other stimuli from the muscle.

To perform these studies Dr. Wilkinson required an in-vitro bath for her apparatus that could permit stretch and stimulation of the muscle, but still allow clear access to the isolated nerve-muscle preparation for recording equipment and electrodes.

SOLUTION

The 809C in-vitro configuration (then still a model 809B) was used to complete these experiments. Dr. Wilkinson was one of the first to use the apparatus in this configuration and the spacious, minimal design allowed easy access to the innervated preparation for attachment of the muscle and most importantly to place the necessary electrodes in order to record from the sensory nerve. Temperature control and means for changing the perfusate solution were also included with the system, enabling convenient manipulation of muscle stimuli.

RESULTS

The chamber was instrumental in the completion of Dr. Wilkinson’s postdoctoral research at Emory. Our design permitted simultaneous nerve and muscle recordings in response to a variety of stimuli. It worked so well that today she uses two instruments in her new laboratory at SJSU. Dr. Wilkinson also used the chamber as part of a recent JoVE methods paper about her fascinating research, as well as highlighting it in many other publications.

809C Helps Neuroscientist Assess Muscle Function in Model of Peripheral Nerve Injury

CHALLENGE

Dr. Young-Jin Son at Shriners Hospitals Pediatric Research Center studies peripheral nerve regeneration and the role Schwann cells play in maintaining and aiding regeneration of synaptic connections. Dr. Son was looking for a way to test functional recovery in mice with peripheral nerve injury in addition to measuring number of motor units and neuromuscular junction (NMJ) properties.

Dr. Son was well-versed in electrophysiological recordings, however, he wanted a way to test muscle function in response to regenerating sensory and motor afferents as well as determining the quality of re-innervation.

SOLUTION

Due to the numerous parameters to be measured and different preparations required, Aurora Scientific knew the 809C apparatus would provide Dr. Son the configurability he needed. The 809C whole mouse apparatus combined with our flagship 300C Dual-Mode lever provided a cost effective way to quickly perform in-situ or in-vivo measurements in mice. Aurora Scientific also included our 809C-25 bath which attaches to the 809C apparatus to enable in-vitro measurement of nerve-muscle preparations.

RESULTS

With Aurora Scientific’s 3-in-1 system, Dr. Son has the ability to perform isometric muscle force measurement in mice, which is known to be an indicator of functional recovery in models of peripheral nerve regeneration. In addition, Dr. Son found great use of our in-situ configuration, which allows him to measure isometric force in specific muscles while maintaining vascular and neural connections. Furthermore, our in-vitro bath has allowed Dr. Son to use a nerve-muscle preparation to measure number of motor units as well as test NMJ properties in these models. The long term goal of his lab is to be able to perform longitudinal studies to measure functional recovery in-vivo in response to various treatment regimes. Aurora Scientific’s 1300A system provides the versatility that Dr. Son requires and will allow him to measure mechanical properties as his research progresses.

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Specifications

Apparatus 809C
Animal Platform Dimensions [mm] 90 W x 115 L
Platform Horizontal Movement [mm] 100
Lever System1 Movement [mm] Vertical: 41
Side-to-side: 125
Resting Tension Adjustment [mm] ±10
Tension Travel per Knob Rotation [mm] Course: 20
Fine: 2.6
Limb Clamp Fixation 26 mounting locations

1 = Compatible with 300C and 305C lever systems

Temperature Control 809C
Type Circulated heated water
Fittings Dry-break, quick connect
General Specifications 809C
Weight [kg] 1.7
Dimensions [cm] Overall: 21 W x 28 L x 12 H
Frame: 21 W x 26 L x 12 H

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