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Commonly Used Samples and Experiments using Small Intact Isolated Muscle Systems

Commonly Used Samples and Experiments using Small Intact Isolated Muscle Systems

By In Blog On November 15, 2019


Aurora Scientific offers several different test systems to evaluate the various structural levels of muscle. However, there are many examples of these systems being used for other types of compliant tissues and materials. Most of these systems are able to work with a multitude of different samples and perform a wide array of experiments.

This blog is meant to be a quick, comprehensive guide to what research has been done with the 1500A/1510A/1530A Small Intact Isolated Muscle System and a primer for anyone searching for the most appropriate muscle test system based on their specific research objectives.

This blog is part of a collection – click below to view other guides by system type:

1200A/1205A Isolated Muscle Systems for Mice and Rats

1300A/1305A/1310A Whole Animals Systems for Mice, Rats, Dogs, Pigs and Other Large Animals

1400A/1405A/1410A/1415A Permeabilized Fiber System

1600A – Permeabilized Myocyte System

1700A – Myofibril System


1500A/1510A/1530A Small Intact Isolated Muscle System

The 1500A series is the most flexible system in terms of what types of samples it can work with.  This system has a small horizontal bath intended for any small skeletal muscles, cardiac and smooth muscle strips, as well as intact single fibers and small bundles.  Mechanical measurements of artificially grown muscle constructs have also been performed with this system.  The types of experiments that are performed with this system are similar to the 1200A and 1300A (in-vitro only) families, however, the 1500A provides additional features and complimentary experiments such as biofluorescence of the tissue as well as the potential to measure oxygen consumption during an experiment.

To download a chart of the information below, click here!

Common Samples:

Skeletal Muscle:

  • Extensor Digitorum Longus (EDL)
  • Soleus
  • Plantaris
  • Diaphragm
  • Lumbricalis
  • Engineered Constructs (iPSC-derived)

Cardiac Muscle:

  • Small bundles or strips
  • Commonly trabeculae or papillary muscle
  • Engineered Constructs (iPSC-derived; sheets, bundles, rings)

Smooth Muscle:

  • Single or small bundle of de-membranated fibers
  • Any muscle can be tested
  • Commonly bladder, colon, vaginal or vascular bundles or fibers

Artificial Muscle and Connective Tissue:

  • Cartilage
  • Epithelial
  • Tendon

Biomaterials and Polymers:

  • Dielectric Elastomer Actuators (EAPs)
  • Electroactive Polymers
  • Gels & Scaffolds
  • Electrochemical Actuators
  • Electrothermal Actuators

Common Experiments:

Twitch Force:

Single impulse designed to elicit contraction of a single or small number of muscle fibers within a bundle.

Common Experiment Data Twitch Aurora Scientific

Tetanic Force:

Multiple electrical impulses in quick succession resulting in temporal summation and complete muscle contraction.

Common Experiment Data Tetanus Aurora Scientific

Force – Frequency:

Varying rates of stimulation frequencies to assess optimal frequency that elicits maximum tetanic force.

Common Experiment Data Force-Frequency Aurora Scientific

Fatigue:

Sub-maximal tetanic contraction repeated frequently to induce fatigue of the muscle.

Common Experiment Data Fatigue Aurora Scientific

Eccentric:

Active lengthening of muscle during isometric tetanic contraction to induce injury and assess resistance to injury or recovery from it.

Common Experiment Data Eccentric Aurora Scientific

Force-Velocity (shortening velocity):

Maximal muscle stimulation followed by a series of force clamps to a percentage of maximum force, allowing for measurement of shortening velocity.

Common Experiment Data Force-Velocity 2 Aurora Scientific

Stiffness:

Passive sinusoidal lengthening and shortening of muscle to assess the inherent stiffness of the tissue.

Common Experiment Data Stiffness Aurora Scientific

Stress – Strain:

Incremental lengthening of tissue to calculate Young’s modulus of materials.

Common Experiment Data Stress-Strain Aurora Scientific

Active Force-Length (Frank-Starling):

Pacing cardiac tissue at a constant or varying frequency while incrementally lengthening the muscle to increase preload in order to assess the excitation-contractile mechanisms.

Common Experiments Data Active Force-Length Aurora Scientific

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