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Commonly Used Samples and Experiments using Whole Animal Systems

Commonly Used Samples and Experiments using Whole Animal Systems

By In Blog On November 4, 2019


Aurora Scientific offers several different test systems to evaluate the various structural levels of muscle. However, there are many examples of these systems being used for other types of compliant tissues and materials. Most of these systems are able to work with a multitude of different samples and perform a wide array of experiments.

This blog is meant to be a quick, comprehensive guide to what research has been done with the 1300A/1305A/1310A Whole Animal Systems and a primer for anyone searching for the most appropriate muscle test system based on their specific research objectives.

This blog is part of a collection – click below to view other guides by system type:

1200A/1205A Isolated Muscle Systems for Mice and Rats

1400A/1405A/1410A/1415A Permeabilized Fiber System

1500A/1510A/1530A Small Intact Isolated Muscle System

1600A – Permeabilized Myocyte System

1700A – Myofibril System


1300A/1305A/1310A Whole Animals Systems for Mice, Rats, Dogs, Pigs and Other Large Animals

Our 1300A system was originally designed solely for performing footplate (in-vivo) experiments in mice and rats. It quickly expanded into a system which could also be used for in-situ experiments and a horizontal in-vitro add on bath was also built. Various versions of the system were later built to accommodate larger animal models up to the size of pigs and dogs. The typical experiments which can be performed are similar to those of the 1200A system family, except that a much broader selection of muscles can be tested by the in-situ method. Those muscles that do not have a surgically accessible tendon or are very large which would have been impossible to test in-vitro are now potentially in play using the in-situ method.

To download a chart of the information below, click here!

Common Samples:

Skeletal Muscle – in-vitro:

  • Extensor Digitorum Longus (EDL)
  • Soleus
  • Plantaris
  • Diaphragm
  • Lumbricalis

Skeletal Muscle – in-situ:

  • Tibialis Anterior (TA)
  • Gastrocnemium
  • Quadriceps
  • Hamstring
  • Tongue
  • Rotator Cuff

Skeletal Muscle – in-vivo:

  • Dorsiflexors (TA, EDL)
  • Plantarflexors (Gastroc, Soleus, Plantaris)
  • Jaw
  • Forefinger (Flexor Digitorum Brevis; FDB)

Artificial Muscle and Connective Tissue:

  • Cartilage
  • Epithelial
  • Tendon

Common Experiments:

Twitch:

Single impulse designed to elicit contraction of a single or small number of muscle fibers.

Common Experiment Data Twitch Aurora Scientific

Tetanus:

Multiple electrical impulses in quick succession resulting in temporal summation and complete muscle contraction.

Common Experiment Data Tetanus Aurora Scientific

Fatigue:

Sub-maximal tetanic contraction repeated frequently to induce fatigue of the muscle.

Common Experiment Data Fatigue Aurora Scientific

Force Frequency:

Varying rates of stimulation frequencies to assess optimal frequency that elicits maximum tetanic force.

Common Experiment Data Force-Frequency Aurora Scientific

Eccentric:

Active lengthening of muscle during isometric tetanic contraction to induce injury and assess resistance to injury or recovery from it.

Common Experiment Data Eccentric Aurora Scientific

Gait Analysis/Modelling Exercise (Isotonic, Concentric):

Controlling muscle force output (isotonic) to assess shortening velocity of muscle.

Common Experiments Data Gait Analysis Aurora Scientific

Stiffness:

Passive sinusoidal lengthening and shortening of muscle to assess the inherent stiffness of the tissue.

Common Experiment Data Stiffness Aurora Scientific

Stress-Strain:

Incremental lengthening of tissue to calculate Young’s modulus of materials.

Common Experiment Data Stress-Strain Aurora Scientific

Looking for more information on Whole Animal Test Systems?

CLICK to learn more about the 1300A Series...