The Video Sarcomere Length (VSL) system provides high-speed, accurate sarcomere length measurement from a live video image. Two systems are available which are distinguished by the camera used to acquire the images. The 900B-VSL provides a low cost accurate capture of sarcomere length using a USB, CMOS camera. This provides full frame images at up to 80 frames per second (640×480) with a maximum speed of 1,000 frames per second for a reduced region of interest (ROI). Our high performance 901B-High-Speed VSL (HVSL) system uses a GigE Ethernet camera to provide full frame images (640×480) at up to 300 frames per second with a maximum of 4,700 frames per second when windowed down.
Our software allows dynamic tracking of sarcomere length within the image which ensures that as the tissue moves and contracts the same sarcomeres will always be used for the calculation. Image capture and sarcomere length data can be controlled by our 600A Digital Controller software which runs on a real-time Linux operating system.
Unique record and playback functions allow high-speed streaming to disk and permit calculation of sarcomere length from image files. The analysis function graphically displays sarcomere length and velocity over time.
The software includes a Scope function that provides a scrolling data display of sarcomere length with time. Researchers can accurately and easily record up to 2 hours of sarcomere length data. The Scope display allows freeze-framing at any time. Operators can then scroll through the sarcomere history and log SL data to disk at rates of up to 1,000 samples per second.
The programs include two length calculation methods, Autocorrelation Function (ACF)/Sine-fit and Fast Fourier Transform (FFT) with curve fit.
Microscope Calibration Slide for 900B/901B
900B-SA: Video Sarcomere Length Standalone System
Standalone video sarcomere length system that includes Linux PC, camera, software and all cables
901B-SA: High-Speed Video Sarcomere Length Standalone System
Standalone high-speed video sarcomere length system that includes Linux PC, high-speed camera, software and all cables
- two systems available: 900B (VSL) low cost, 1000 frames per second, 901B (HVSL) – high performance, 4700 frames per second
- dynamic tracking of region of interest – keeps the same sarcomeres in the region of interest as the tissue moves and contracts
- real-time sarcomere length measurement from digital video camera
- calculates sarcomere length accurately even with small sample regions and poor image quality
- easy calibration (Field of View or Calibrated Length)
- two length calculation methods: FFT and Autocorrelation Function/Sine-fit
- complete system including: HVSL Software, PC, high-speed CMOS camera, camera power supply, all required cables
- real-time scrolling data display, stores up to 2 hours of sarcomere length data, can also log data to disk at up to 1000 samples per second
- record and playback functions that allow high-speed streaming to disk and calculation of sarcomere length from image files
- SL control, image capture and SL data output can be controlled by 600A Digital Controller
900B – Sarcomere Length in Zebrafish Larvae
In 2011 Dr. Dennis Claflin approached us to try out our 900B Video Sarcomere Length system. Dr. Claflin was a long time user of Aurora Scientific equipment and an expert in single fiber measurements. He had been using a simple laser diffraction method to quantify SL in his permeabilized fibers, however, this method was not compatible with another one of his chambers.
This chamber would be mounted on an inverted microscope and used to test the mechanical forces of single, living zebrafish larvae. Because of their relative ease of rearing and their transparent body, these larvae can be used as an inexpensive model for a variety of human myopathies.
Since our 900B Video Sarcomere Length system uses a c-mount camera, it is compatible with virtually all inverted microscopes. Additionally, there are no complicated procedures for calibration of different tissues. A simple reticule to correlate camera pixels to distance and 5 minute calibration was all that was required. As long as the sample presents a remotely visible sarcomere pattern the software would converge to the dominant solution. All of these attributes made the 900B the right choice to study zebrafish larvae.
The video sarcomere length system was put to work on a variety of different tissues such as lumbrical muscle and ultimately zebrafish. The camera proved quite successful for setting resting SL in the zebrafish larvae despite the overlapping of tissue and skin. The lab’s results using these methods have been extensively published and were recently featured in a JoVE video publication.
Models In The Series
Accurate and low cost real-time calculation of sarcomere length in muscle tissue from video camera.