1305B

Integrated 3-in-1 Whole Animal System for Rats

The 1305B integrated 3-in-1 muscle system is a leading solution for quantifying mechanical properties of muscle in rats. This system utilizes the power of our long standing 3-in-1 muscle system and creates a smaller footprint for labs with limited or non-permanent space.

By combining in situ, in vivo, and in vitro muscle tests using one simple platform, researchers are able to capture a complete picture of muscle physiology in rats. It is ideal for a number of hindlimb skeletal muscles including the EDL, soleus, tibialis anterior (TA), gastrocnemius and quadriceps. The system comes with a rat apparatus, complete with temperature controlled animal and limb plate designed to support and fix the animal and limb being tested. In addition, the system can be converted to an isolated (in vitro) muscle test system with the attachment of an optional 25mL horizontal muscle bath for measurement of EDL, soleus, diaphragm and other smaller muscle samples.

Aurora Scientific’s flagship Dual-Mode lever system is packaged into a powerful Integrated Test System along with our High-Power Bi-Phasic Stimulator and USB Data Acquisition hardware. This enables the same power and functionality of our long-standing 1305A system, but in a smaller footprint with minimal cabling that makes it portable and laptop controllable. Conveniently, muscle samples are attached at only one point to measure force and length, saving time and
increasing productivity.

Rat or larger rodent limb plate for in-situ experiments

Rat or larger rodent foot plate for in-vivo protocols

Horizontal bath for in-vitro protocols

powerful 3-in-1 design: save time and space while increasing productivity
small footprint and easy portability for limited lab spaces
ideal for live animal protocols: precision platform with temperature control
high experiment throughput

fast data acquisition and analysis software on a convenient Windows Laptop
dual purpose field/nerve stimulator
capable of dynamic functional measures in addition to isometric
functional measures from EDL, SOL, TA, GA, Diaphragm & more!

Tell the Whole Story – Live Animal & Isolated Tissue in One

The three system configurations allows the researcher to work with a broad range of muscle types, providing a convenient platform for compound screening, phenotype evaluation and comparison of murine models; all within one system.

High Throughput Software Capability

Experience high-throughput data analysis, including batch processing and multi-parameter calculations for hundreds of muscle samples within minutes. Downstream analysis can be completed within Aurora Scientific DMC/DMA software or exported to your analysis program of choice.

650A: Real-Time Muscle Data Acquisition and Analysis Software

Standard Protocol Library

The protocol library includes a variety of muscle experiments for rat in vitro studies. Protocols include system operation and data acquisition settings optimized for sample type and measurement needs. Add your own custom protocols as well to streamline system operation with multiple lab members.

Stories of Success

1305A – Footplate Configuration for Rats Invaluable to the Study of Muscle Damage

CHALLENGE

In 2012, Marius Locke at the University of Toronto had been studying skeletal muscle damage and the role that heat shock proteins (HSPs) play in protecting the muscle from stress induced damage, such as exercise. Dr. Locke was looking for equipment that could reliably and repeatedly induce muscle damage in his rats while accurately quantifying force production and recovery.

SOLUTION

Our 1305A system was suggested for the job as it had the ability to perform the kinds of repeated contractions that Dr. Locke was looking to accomplish. It was first implemented piecemeal to integrate with some existing equipment in the laboratory, but because of its ease of use, the remaining Aurora Scientific pieces were added for a complete system. Aurora Scientific technical support helped craft the best protocols for Dr. Locke’s experiments and soon thereafter the first cohort of rats was tested.

RESULTS

The system has helped Dr. Locke and his graduate students publish valuable findings about the protective role of HSPs in damaged muscle. In addition, Dr. Locke established an important rat model of standard human exercise protocols to gain valuable, physiologically relevant data which may one day be applicable to athletes and people alike.

Select References

Charles et al. “The impacts of muscle-specific force-velocity properties on predictions of mouse muscle function during locomotion” Frontiers in Bioengineering and Biotechnology (2024) DOI: 10.3389/fbioe.2024.1436004
Segales et al. “Sestrin prevents atrophy of disused and aging muscles by integrating anabolic and catabolic signals” Nature communications (2020) DOI: 10.1038/s41467-019-13832-9
Gorini et al. “Reversible temporally-specific inhibition of muscle using a light-activated chloride channel” bioRxiv (2018) DOI: 10.1101/398156
Cosentino et al. “Remodeled eX vivo muscle engineered tissue improves heart function after chronic myocardial ischemia” Scientific Reports (2023) DOI: 10.1038/s41598-023-37553-8
Ruud et al. “Regulation of cardiomyocyte t-tubule structure by preload and afterload: Roles in cardiac compensation and decompensation” The Journal of Physiology (2024) DOI: 10.1113/JP284566
Llewellyn, Michael E. et al. “Orderly recruitment of motor units under optical control in vivo.” Nature Medicine (2010) DOI: 10.1038/nm.2228
Ma et al. “Myosin Head Configurations in Resting and Contracting Murine Skeletal Muscle” Internation Journal of Molecular Sciences (2018) DOI: 10.3390/ijms19092643
Hamoudi et al. “Muscle weakness and selective muscle atrophy in osteoprotegerin-deficient mice” Human Molecular Genetics (2020) DOI: 10.1093/hmg/ddz312
Delfinis et al. “Muscle weakness and mitochondrial stress occur before severe metastasis in a novel mouse model of ovarian cancer cachexia” Molecular Metabolism (2024) DOI: 10.1016/j.molmet.2024
Tatarenko et al. “Multiscale analysis of Klf10’s impact on the passive mechanical properties of murine skeletal muscle” Journal of the Mechanical Behavior of Biomedical Materials (2024) DOI: 10.1016/j.jmbbm.2023.106298

System Components

Interested in the 1305B Integrated Whole Animal System for Rats?

Connect with us to discuss your research, schedule a demo or request a quote!

Resources

Parts & Accessories

Model #	1305A
300-3B	3 cm long replacement lever arm, attaches to 300E or older 300B/C series motors for in-vitro and in-situ measurement
300-4B	4 cm long replacement lever arm, attaches to 305E or older 305B/C series motors for in-vitro and in-situ measurement
300-CLA	Custom length replacement lever arm, attaches to 300/305E or older 300B/C and 305B/C series motors for in-vitro and in-situ measurement
300-CBL-3	3m replacement motor cable (other lengths available on request)
300-CBL-FR	0.4m long cable to reverse force direction of 300E/305E motor
300E-MOTOR	Replacement motor for 300E dual-mode lever system (Lever arm sold separately)
305E-MOTOR	Replacement motor for 305E dual-mode lever system (Lever arm sold separately)
603D	16-bit NI X Series USB A/D with interface
603E	16-bit NI X Series PCI express A/D card
606A	(2) 150cm long BNC to BNC cables
607A	50 Ohm BNC terminator to minimize channel crosstalk within signal interface
615B	2nd Copy of DMC and DMA suite software
615C	DMC and DMA suite software upgrade
808-1	Vertical pin clamp that utilizes a 27G hypodermic needle to immobilize the mouse knee joint during in-situ experiments. It mounts on our family of 3-in -1 apparatus.
808-2	Vertical Screw Clamp that uses sharpened, stainless steel screws and a Delrin pad to fix mouse knee joint during in-situ experiments. It mounts on our family of 3-in-1 apparatus.
808-4	Upright knee clamp designed for mouse or rat that uses a dowel pin with a pad on the end for gentle immobilization of rat knee during in-vivo or in-situ experiments. It mounts on our family of 3-in-1 apparatus.
808-5	Upright knee clamp designed for mouse that uses a horizontal, sharpened stainless steel screw to prevent hind limb/joint movement during in-vivo or in-situ experiments. It mounts on our family of 3-in-1 apparatus.
808-6	Upright knee clamp designed for mouse that uses a horizontal dowel pin with pad for non-invasive knee restraint during in-vivo or in-situ experiments. It mounts on our family of 3-in-1 apparatus.
808-7	Larger upright knee clamp designed for large rats that uses a dowel pin with a pad on the end for gentle immobilization of rat knee during in-vivo or in-situ experiments. It mounts on our family of 3-in-1 apparatus.
809-CBL-EA	Male BNC to female touch proof adapter cable to connect a BNC cable to needle electrodes
809-ELEC	EMG style needle electrodes with touch proof connector (10 pack) for in-vivo and in-situ stimulation
809-ELEC-EEG	EEG style needle electrodes with touch proof connector (10 pack) for in-vivo and in-situ stimulation
809C-25	Horizontal muscle bath which replaces the animal platform on 809C apparatus for in-vitro intact muscle mechanics experiments. The bath couples to the apparatus by removing the 4 screws for the limb platform and using them to attach the bath in its place. The volume of the bath is 25mL. Tools, tubing and fittings are also provided.
801-CBL-EA	Male BNC to Male touch proof adapter cable to connect a BNC cable to the built-in electrodes on the 809C-25
801-RES	1L reservoir assembly complete with built in, oxygenating bubbler. The reservoir is meant to supply oxygenated media to the 809C-25. The reservoir has an outer glass water jacket and can be connected to a circulating water reservoir to maintain perfusate temperature. Tubing and fittings to couple the reservoir are provided.
830A	Half-rack adapter that allows half-rack instruments to be mounted in standard 19” racking. Works with model 701C
831A	Heavy gauge, steel desktop or shelf-top rack, with standard 19” width for any Aurora Scientific Instruments. Available in various heights. (6U high for 1200A and 1300A, 5U high for 1400A, 1500A and 1600A systems).