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955A: FluoroTrack – Calcium Fluorescence Software

FluoroTrack is powerful, twitch-based, software that enables physiology researchers to easily measure intracellular calcium along with tissue length, force and sarcomere length. The software seamlessly integrates with all Aurora Scientific products and with fluorescence photometry equipment from leading suppliers. The software can be run in manual our automatic modes, providing the researcher with maximum flexibility when trying new protocols or executing repeated complex experiments.

The 955A FluoroTrack software controls the fluorescence photometry equipment and collects the fluorescence signals from the photomultiplier. Test protocols written using the 600A Protocol Editor control the entire experiment including the stimulator, high-speed length controller, force transducer, apparatus, fluorescence equipment and sarcomere length measurement. Data collection from all of these instruments is stored in a single, time-stamped, file. This highly integrated system allows a single computer to control and store data from many different instruments including those from Aurora Scientific, IonOptix and Sutter.

Powerful Twitch Analysis provides an easy-to-use analysis and data export capability. Length, Force, SL, Ratio and Calcium data streams can be analyzed to provide average twitch data and statistics.

  • integrates with various Aurora Scientific systems to provide simultaneous acquisition of fluorescence photometry and tissue length, force, and sarcomere length
  • controls all Aurora Scientific instruments plus light sources from major manufacturers (IonOptix HyperSwitch and Sutter Lambda DG-4) – automate your experiment
  • twitch based data acquisition and analysis ideal for cardiac studies (includes stimulator control)
  • calculate ratio or Calcium (Ca2+) concentration at up to 1000 samples per second
  • real-time scrolling display of all data channels
  • automatic storage of all data in a single, time synchronized data file with user specified tags to note important experimental events – never lose your data

950A – Software Module Helps Synchronize Cutting Edge Data In Myocyte Research

CHALLENGE

In late 2013 Dr. Chris Ward of the University of Maryland was working on improving experimental techniques for measuring force, SL and calcium release in single, living myocytes from the FDB muscle.  Dr. Ward had been a long time user of our equipment and was already using our 600A and HVSL software programs to record force and SL.  However, he had been using another lab built solution to measure calcium and was searching for a way to easily synchronize all three measurements.

SOLUTION

The Aurora Scientific FluoroTrack software proved to be the missing piece.  FluoroTrack allowed real-time monitoring of calcium transients and integrated seamlessly with the existing HVSL and 600A programs. In addition, Aurora Scientific made minor customizations to be compatible with Dr. Ward’s existing light source and calcium measurement instruments.

RESULTS

After making these configurations Dr. Ward was able to begin collecting synchronized data almost immediately. This integration provided a single, time-stamped file for easy collection and analysis of force, SL and calcium from FDB cells. The data collected were presented at a Biophysical society meeting some short 2 months later and has gone on to be published.  The system is still an integral part of Dr. Ward’s research and the software continues to evolve and be improved thanks to feedback from Dr. Ward’s lab and other researchers.

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